
Aarti Chaturvedi, Ritu Garg, Varsha A Singh
Evaluating the Correlation of Different Culture Media for the Identification and Isolation of Common Gram Positive Uropathogens and Yeast Causing UTI
Int.J.Adv.Microbiol.Health.Res.2018; 2(3):47-50
Publisher: IJAMHR, Category: Current Issues
Abstract
Background: Urinary tract infection (UTI) is a common problem diagnosed and treated in
urgent care medicine practice. Urinary tract infection (UTI) is a collective term that
describes any infection involving any part of the urinary tract, namely the kidneys, ureters,
bladder and urethra. Clinicians need to be aware of the advantages and limitations of
diagnostic tests. Urine cultures occupy most of the workload of routine microbiology
laboratories in developing country . Accurate and rapid identification of pathogens is
important for a clinical microbiology laboratory. Materials and Methods: Mid-stream
urine and catheterized samples were collected. Cultures were plated on blood agar,
MacConkey agar and cysteine lactose electrolyte deficient media and incubated overnight at
35°C-37°C in ambient air. Colonies on the MacConkey agar, CLED agar and blood agar
were also identified. The final identification of the isolates was done using standard
identification protocol. Results: Out of 500 urine samples processed, 199 showed pure
growth .Out of 199 pure growths, 56 were gram positive cocci and 17 were yeast. Out of all
gram positive cocci showed growth but most of the gram-positive cocci and yeast were
unable to grow on Mac-Conkey agar and blood agar but grew successfully on CLED agar.
Conclusions: So, in resource constrain laboratories, CLED agar can be used as media of
choice for isolation of common uropathogens , cost effective and decreases work load of the
laboratories.
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