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Call for Research / Review Articles


International Journal of Advance Microbiology and Health Research (IJAMHR)

I hereby want to invite you to submit a paper for the International Journal of Advance Microbiology and Health Research (IJAMHR).

We are pleased to inform you that we are started a new journal on health sciences and we are in the process of releasing our First Issue of First Volume of our journal. We would be grateful if you would submit a paper for to complete the first issue of first volume. Research Article, Review Article, Case Reports, and Brief Communication etc are welcome for possible publication in this issue.

It would be grateful if you could submit your paper on or before 15 September, 2017. If you are ready with the manuscript please submit your article at editorijamhr@gmail.com

Looking forward to hear from you soon
Thanks & Regards
Editor In-Chief
International Journal of Microbiology and Health Research


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Sahar Yaslianifard, Mehdi Mirzaii, Ali Taheriniya, Shokoofeh Zamani, Zeynab Abdi Zamharir, Babak Behnam, Mohammad Mehdi Soltan-Dallal, Alireza Soleimani, Somayeh Yaslianifard

Detection of Mex Efflux Pumps in Pseudomonas aeruginosa using a Multiplex Phenotypic and Genotypic
Int.J.Adv.Microbiol.Health.Res.2018; 2(1):1-16

Publisher: IJAMHR, Category: Current Issues



Abstract

Background: The multidrug resistance phenotype of Pseudomonas aeruginosa is largely attributable to expression of Mex efflux pumps. Detection of such pumps expression in different clinical setting would help to select the best antibiotic treatment of choice. Materials and Methods: A multiplex phenotypic and genotypic method was applied for the differential recognition of resistance mediated by the four major transporters, including MexAB-OprM, MexCD-OprJ, MexEF-OprN, andMexXY-OprM. The multiplex approach was performed on 40 selected clinical isolates. Minimum Inhibitory Concentration (MIC) method was respectively used for phenotypic detection using reporter antibiotics Carbenicillin,Erythromycin,Norfloxacin,Imipenem and gentamicin. Genotype was detected using semi-quantitative and quantitative competitive real-time PCR of mex C and mex Eandmex A and mex X, respectively. Both methods were relevant with good levels of overexpression. Results: There were compare and research between phenotypic and genotypic methods in control strains for allmentioned pumps. Among clinical isolates, similarity was observed in 28 out of 40 strains for MexAB-OprM and MexCD-OprJ, and in 32 out of 40 strains for MexXY-OprM and MexEF-OprM. Conclusion: Our results validate the multiplex phenotypic and genotypic as a suitable methodsfor the diagnosis of resistance mediated by four Mex efflux pumps in P. aeruginosa and consequently application of the correct antibiotic.


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