Call for Research / Review Articles

International Journal of Advance Microbiology and Health Research (IJAMHR)

I hereby want to invite you to submit a paper for the International Journal of Advance Microbiology and Health Research (IJAMHR).

We are pleased to inform you that we are started a new journal on health sciences and we are in the process of releasing our First Issue of First Volume of our journal. We would be grateful if you would submit a paper for to complete the first issue of first volume. Research Article, Review Article, Case Reports, and Brief Communication etc are welcome for possible publication in this issue.

It would be grateful if you could submit your paper on or before 15 September, 2017. If you are ready with the manuscript please submit your article at editorijamhr@gmail.com

Looking forward to hear from you soon
Thanks & Regards
Editor In-Chief
International Journal of Microbiology and Health Research

Developed By : Hashtag Solutions

Geetha PV, Aishwarya K, Shanthi M, Uma Sekar

Plasmid Mediated Fluoroquinolone Resistance Determinants in Clinical Isolates of Escherichia coli
Int.J.Adv.Microbiol.Health.Res.2019; 3(2):1-6

Publisher: IJAMHR, Category: Current Issues


Introduction: Fluoroquinolones are among the most commonly prescribed antimicrobials because of their broad-spectrum antimicrobial activity. The major mechanisms of fluoroquinolones resistance are plasmid mediated quinolone resistance. However, fluoroquinolone resistance in gram-positive and gram-negative bacteria has emerged and increased globally.> Aim: The aim of the current study was to investigated the frequency of qnr genes and aac(6')-Ib-cr gene, among the ciprofloxacin resistant clinical isolates of Escherichia coli. Materials and Methods: The study included 150 E. coli clinical isolates. Disc diffusion technique was used to determine the co-resistance to other antimicrobials. Minimum inhibitory concentration (MIC) was determined by agar dilution method for ciprofloxacin. The presences of qnr genes and aac(6')-Ib-cr gene were screened by PCR. Transfer of the plasmids for qnr positives was studied by conjugation assay. Results: The rates of resistance to the other antibiotics were: ampicilin (92%), cefotaxime (89%), piperacillin/tazobactam (64%), imipenem (11%) and amikacin (32%). MIC of ciprofloxacin ranged from 4μg/ml to ≥ 256μg/ml. Twenty-one (14%) isolates harbored qnr genes of which qnrB was detected in 12 (8%) and eight isolates (5.3%) harbored qnrS. In one isolates (0.6%) both qnrA and qnrS co-existed. The acc(6’)-Ib-cr gene was found in 118 (78.6%) isolates. Among 21 qnr determinants 16 (76%) transconjugants were successfully transferred. Conclusion: In our study the prevalence of acc(6’)-Ib-cr gene in E. coli is found to be significant.


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